Tetrode Array Neurophysiology: Experimental Methods and Data Analysis

Extracellular recording using adjustable tetrode arrays is a cornerstone of modern systems neuroscience, unlocking how neural circuits encode memory, learning, and behavior. This text-based course guides you step-by-step through the core principles, experimental setups, and modern signal-processing pipelines used in electrophysiology. You will progress from understanding basic neurophysiological concepts to designing recording protocols and exploring how researchers analyze complex neural ensemble data. What you'll learn: Understand the biophysical principles of extracellular recording and tetrode array design; Explore surgical preparation, electrode positioning, and patterned microstimulation protocols; Learn modern spike sorting techniques to isolate single-unit activity from raw voltage signals; Analyze neural population data to study learning and memory mechanisms; Apply modern open-source data standards like Neurodata Without Borders (NWB) to organize neurophysiology datasets. The course begins with foundational neurophysiology concepts and hardware configurations before transitioning into practical experimental methodologies, signal processing, and data analysis strategies. Designed for beginners in neuroscience, biology, or biomedical engineering, this course requires no prior laboratory experience. Begin your journey into systems neuroscience and start decoding brain activity today.